用Mac ELISA法检测病毒性心肌炎患者血清中柯萨奇B组病毒IgM抗体

中华实验和临床病毒学杂志 2000年第4期第14卷 病毒诊断

作者：朱晖张礼璧

单位：中国预防医学科学院病毒学研究所诊断一室北京100052

关键词：柯萨奇病毒B组；心肌炎；酶联免疫吸附测定；IgM

【摘要】目的了解北京地区病毒性心肌炎的病毒感染状况。方法用IgM抗体捕捉ELISA(Mac ELISA)，检测193例临床诊断为病毒性心肌炎患者血清中的Cox B 1～6型病毒特异性IgM抗体。结果Cox B病毒IgM抗体阳性检出率为69.9%(135/193)，其中阳性检出率最高为B3型，占40%(54例)；其余依次为B2型，占29.6%(40例)；B4型占18.5%(25例)；B1型占7.4%(10例)；B5型占3.0%(4例)；B6型占1.5%(2例)。而181例同年龄组非心肌炎患者IgM抗体阳性检出率为20.4%(37/193)。病毒性心肌炎患者血清中的Cox B IgM抗体阳性检出率明显高于非心肌炎患者，两者差异有显著性，U＝9.56，P＜0.01。结论Cox B感染是病毒性心肌炎的主要病因。

Detection of Coxsackie B virus IgM antibody in myocarditis patients by Mac ELISA

ZHU Hui, ZHANG Libi

【Atract】ObjectiveTo detect Coxsackie B virus type 1-6 IgM antibodies from 193 clinically diagnosed myocarditis cases. MethodsThe capture IgM ELISA (Mac ELISA) test wa used. ResultsThe total positive rate of Coxsackie virus B 1-6 IgM antibody in myocarditis patients was 69.6% (135 cases), among which Cox B3 type was as high as 40% (54 cases) of the total positive patients. The others were 29.6% (40 cases),18.5% (25 cases),7.4%(10 cases),3.0% (4 cases), 1.5%(2 cases) of Cox B2,Cox B4,Cox B1,Cox B5 and Cox B6 reectively. Meanwhile only 20.4% (37 cases) Cox B IgM positive cases could be detected in 181 non-myocarditis patients of same ages. The results showed that the positive rate of Cox B virus IgM antibody in clinically diagnosed myocarditis was significantly higher than that in control group (P＜0.01). ConclusionThe Cox B viruses may be the main agents causing viral myocarditis in Beijing cases. Mac ELISA is an easy， rapid，ecific and seitive method to detect Cox B virus IgM antibody for early and rapid diagnosis of myocarditis.

【Key words】Coxsackie B viruseMyocarditiEnzyme-linked immunosorbent aay;IgM

柯萨奇B组病毒(Cox B)隶属于小RNA病毒科，其感染可引起人类病毒性心肌炎、无菌性脑膜炎、新生儿全身性感染等多种疾膊1〕。据近年来报道急性病毒性心肌炎和心包炎多数情况下是由柯萨奇B组病毒引起的。这类感染在免疫功能正常的患者最初临床症状易被忽视，一部分病例可以自愈，但也可能发展成严重的症状〔2〕。临床上主要靠检测特异性IgM抗体分析其可能病因。本文即报道了Cox B 1～6型IgM抗体的检测结果。